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Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
Primary Antibodies Against Etar #Sc 33536, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
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Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
Bacillus, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
Et A Sc 33536, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v damsela atcc 33536  (ATCC)
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ATCC v damsela atcc 33536
Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
V Damsela Atcc 33536, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prevotella nigrescens atcc 33536  (ATCC)
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ATCC prevotella nigrescens atcc 33536
Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: <t>ETAR</t> expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. <t>II:</t> <t>ETBR</t> expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.
Prevotella Nigrescens Atcc 33536, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: ETAR expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. II: ETBR expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.

Journal: Urology Research and Practice

Article Title: Altered Endothelin Receptor Expression in Idiopathic Male Infertility: A Potential Therapeutic Target

doi: 10.5152/tud.2025.24104

Figure Lengend Snippet: Immunofluorescence staining of endothelin receptors in normozoospermic semen. I: ETAR expression: I.A, ETAR expression (green) is localized to the plasma membrane surrounding the post-acrosomal region of the sperm head. I.B, Sperm DNA (blue, stained with DAPI) is confined to the head. I.C, the merged image confirms colocalization of ETAR and DNA signals in the head region. II: ETBR expression: II.A, ETBR expression (green) is observed throughout the post-acrosomal area of the sperm head. II.B, DAPI staining (blue) highlights the sperm nucleus. II.C, the merged image demonstrates overlapping localization of ETBR and nuclear staining. Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole, ETAR, endothelin receptor type A; ETBR, endothelin receptor type B.

Article Snippet: A 10 μL aliquot was applied to glass slides (Thermo Fisher Scientific, Germany) and incubated with primary antibodies against ETAR (#sc-33536) and ETBR (#sc-518149; both from Santa Cruz Biotechnology, USA) at 1 : 1000 dilution for 1 hour.

Techniques: Immunofluorescence, Staining, Expressing, Clinical Proteomics, Membrane

Protein expression levels of endothelin receptors in semen samples. ETAR and ETBR protein expression levels in semen samples were assessed using Western blotting (A) and ELISA (B) in both fertile (normozoospermia) and infertile males (with OAT syndrome and teratozoospermia). A, Strong ETAR expression was found in normozoospermic controls and in a subset of patients with OAT syndrome, whereas ETBR was undetectable in all samples. Detection of GAPDH served as a loading control. B, Increasing ETAR expression levels in semen of fertile (normozoospermia) men compared to infertile participants (Kruskal-Wallis test, p < 0.05). ETBR expression showed no significant differences between groups. Two distinct ETAR expression patterns were identified within the OAT group. Abbreviations: ETAR, endothelin receptor type A; ETBR, endothelin receptor type B; OAT syndrome, oligo-astheno-teratozoospermia.

Journal: Urology Research and Practice

Article Title: Altered Endothelin Receptor Expression in Idiopathic Male Infertility: A Potential Therapeutic Target

doi: 10.5152/tud.2025.24104

Figure Lengend Snippet: Protein expression levels of endothelin receptors in semen samples. ETAR and ETBR protein expression levels in semen samples were assessed using Western blotting (A) and ELISA (B) in both fertile (normozoospermia) and infertile males (with OAT syndrome and teratozoospermia). A, Strong ETAR expression was found in normozoospermic controls and in a subset of patients with OAT syndrome, whereas ETBR was undetectable in all samples. Detection of GAPDH served as a loading control. B, Increasing ETAR expression levels in semen of fertile (normozoospermia) men compared to infertile participants (Kruskal-Wallis test, p < 0.05). ETBR expression showed no significant differences between groups. Two distinct ETAR expression patterns were identified within the OAT group. Abbreviations: ETAR, endothelin receptor type A; ETBR, endothelin receptor type B; OAT syndrome, oligo-astheno-teratozoospermia.

Article Snippet: A 10 μL aliquot was applied to glass slides (Thermo Fisher Scientific, Germany) and incubated with primary antibodies against ETAR (#sc-33536) and ETBR (#sc-518149; both from Santa Cruz Biotechnology, USA) at 1 : 1000 dilution for 1 hour.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Control